Induksi Kalus dengan BAP (Benzylaminopurin) dan IAA (Indoleacetic acid) pada Tanaman Cabai (Capsicum annuum L.) Lokal Genotipe Lotanbar Sumatera Barat
Authors
Saipul Sihotang , Renfiyeni Renfiyeni , Irfan Suliansyah , Jamsari JamsariDOI:
10.31289/agr.v3i2.2547Published:
2019-06-13Issue:
Vol. 3 No. 2 (2019): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, JuniKeywords:
Induction, In Vitro, Genotype, Lotanbar, CallusAbstract
This study was aimed to obtain the method in the study of callus induction of in vitro. The research activities were carried out at Laboratory of Biotechnology, Faculty of Agrotechnology, Andalas University. This research used decriptive and experimental methods. One-way ANOVA analysis followed by Duncan’s test was used to determine significant differences (P ≤ 0.05). All statistical analysis were performed using the SPSS Ver. 23 statistical software package. The results showed that medium of callus induction is 4,0 mg/L BAP + 0,5 mg/L IAA with formation of callus (days), and average of callus formed, namely 4,33 days, and 9,67 callus.References
Agrawal, S. Chandra, N. dan Kothari, S. L. (1989). Plant Regeneration In Tissue Cultures Of Pepper (Capsicum annuum L. cv. mathania). J. Plant. Cell. Tis. and Org. C. 16: 47–55.
Ahmad, I., B, Mahir, M., N, and Zaiton, A, (2002). Transformation of Red Chili Cilibangi-2 (Capsicum annuum L.) with CDNA of Cucumber Mosaic virus protein gene by direct Uptake. Pakistan Journal of Biological Science 5 (6): 683-687.
Badan Pusat Statistik Tanaman Sayuran dan Buah-buahan Semusim. (2016). Luas Panen, Produksi dan Produktivitas Cabai.
FAO (Food and Agriculture Organization). (2017). Beberapa Negara dengan Produksi Cabai Tertinggi Dunia. http://jesl.detik.com.ac.id/index.php/ jfiti/article/view/6787/5231. Diakses Jumat 6 Januari 2017.
Faizah, R. (2012). Ketahanan Biokimia Tanaman Cabai Terhadap Begomovirus Penyebab Penyakit Daun Keriting Kuning. J. Fito.8: 138–144.
Faquet, C. M, Mayo, M. A., Maniloff, J. Desselberger, U. dan Ball, L. A. Virus Taxonomy, VIIIth Report of the ICTV. (2005). London (UK): Academic Press.
Han, E, Goo, Y., Lee, M, Lee, S. (2015). An Efficient Transformation Method of Potato var Atlantic. J. Plant Biotechnol. 42: 77-82.
Jamsari, J. Ferita, I. Noverta, A. Husada, E. D., Herberg, F. W., Nellen, W. dan Syukriani, L. (2016). A Pathogenic Isolate of Monopartite PepYLCV DNA A-like Genome Differs Significantly in C1 Gene and CR Sequence, but not in Their other Genes. J. Plant Pathol. 15: 124-134
Kementerian Pertanian. (2015). Luas Panen, Produksi dan Produktivitas Cabai. 2009.
Mansoor, S, Khan, S., H, Bashir, A, Saeed, M, Zafar, Y, Malik, K., A, Briddon, R, Stanley, J, and Markham, P., G. (1999). Identification of a Novel Circular Single-Stranded DNA Associated with Cotton Leaf Curl Disease in Pakistan199: 190–199.
Mansoor, S. Zafar, Y, Briddon, R., W. (2006). Geminivirus disease complexes; the threat is spreading. Trends. Plant. Sci. 11: 209-212.
Manzila, I., Hidayat, S. H., Mariska, I. dan Sujiprihati, S. (2010). Pengaruh Perlakuan Ethyl Methane Sulfonate pada Tanaman Cabai (Capsicum annuum L .) dan Ketahanannya terhadap Chilli Veinal Mottle Virus (ChiVMV) The Effect of Ethyl Methane Sulfonate on Chilli Pepper (Capsicum annuum L.) and Their Resistance to Chil, J. Agro. Bio. I., 38: 205–211.
Nadeak, H., (2016). Perakitan Tanaman Kentang (Solanum tuberosum L.) Kultivar Nooksack Transgenik yang Mengandung Gen Hd3a. Tesis. Institut Pertanian Bogor.
Nasution, P. (2016). Benzyl Amino Purine (BAP) dan Napthaleneacetic Acid (NAA) Mempengaruhi Pertumbuhan Planlet Nenas (Ananas comosus (L.)Merr) Pada Media MS secara In vitro. Skripsi Program Studi Agroekoteknologi Fakultas Pertanian. Universitas Muhammadiyah Sumatera Utara.
Pedri, J, and Jamsari. (2013). Urutan Nukleotida lengkap DNA A-seperti Genome dan DNA- β dari monopartite Lada Yellow Leaf Curl Virus , A annuum Dominan Begomovirus yang menginfeksi Capsicum di West Sumatera Indonesia. Page : 1–14.
Ramchandra, S., and Science, P., S, (2015). Optimization Of Callus And Cell Suspension Culture Of Capsicum Annum. Int J Pharma Bio Sci, page: 664–671.
Renfiyeni, Yusniwati, Trisno, J, dan Jamsari. (2015). Calli Induction Of Some Chili Pepper (Capsicum annuum L.) Genotypes As Material For Genetic Transformation, Andalas University, 1: 75–80.
Renfiyeni. (2015). Studi Regenerasi Dan Transforomsi Genetik Gen Coat Protein Dan Beta Component Geminivirus melalui Agrobacterium tumefaciens pada Tiga Genotipe Cabai (Capsicum annuum L). Pascasarjana Universitas Andalas.
Santoso, T., J. (2008). Identifikasi Begomovirus Indonesia Pada Tomat Dan Analisis Diversitas Genetik Gen Av1 Serta Pemanfaatannya Untuk Pengembangan Tanaman Tahan Virus Indonesia Pada Tomat Dan Analisis Diversitas Genetik Gen Av1. Disertasi. Institut Pertanian Bogor.
Sihotang, S. (2016). Stimulasi Tunas Pisang Barangan (Musa acuminata L.) secara In Vitro dengan Berbagai Konsentrasi IBA dan BAP. Skripsi. Universitas Medan Area.
Sihotang, S. (2019). Studi Optimasi Regenerasi In Vitro dan Transformasi Genetik DNA satelit Geminivirus menggunakan Agrobacterium tumefaciens pada cabai (Capsicum annuum L.). Tesis. Universitas Andalas.
Sudiono, N, dan Yasin, S., H., H, (2005). Penyebaran Dan Deteksi Molekuler Virus Gemini Penyebab Penyakit Kuning Pada Tanaman Cabai Di Sumatera, Jurnal Tropika, 5(2), hal. 113–121.
Sulandari, SRI, Suseno, R., Hidayat, S., H, Harjosudarmo, J., dan Sosromarsono, S., (2006). Deteksi dan Kajian Kisaran Inang Virus Penyebab Penyakit Daun Keriting Kuning Cabai,†HAYATI Journal of Biosciences. Institut Pertanian Bogor, 13(1): 1–6.
Widiarti, W. (2016). Transformasi Genetik Tanaman Kentang (Solanum tuberosum) kultivar Jala Ipam dengan Gen Hd3a. Tesis. Institut Pertanian Bogor.
Wulandari, D. (2015). Rekayasa Genetik Nicotiana tabacum Kultivar Sr1dengan Gen Peroksidase (Perl) Dari Kedelai Kultivar Lumut. Tesis. Institut Pertanian Bogor.
Verma, S. Dhiman, K. dan Srivastava, D. K. (2013a). Agrobacterium-Mediated Genetic Transformation of Bell Pepper (Capsicum annuum L . Cv . California Wonder). Inter J. of Advan. Biotech. R. 4 : 97-403
Verma, S. Dhiman, K. dan Srivastava, D. K. (2013b). Efficient In Vitro Regeneration From Cotyledon Explants in Bell Pepper (Capsicum annuum L. cv. California Wonder). Inter. J. of Adv. Biotec. and R. 4 : 391-396.
Yang L, Hu W, Xie Y, Li Y, dan Deng Z, (2016). Scientia Horticulturae Factors affecting Agrobacterium -mediated transformation efficiency of kumquat seedling internodal stem segments, 209: 105–112..
Zulkarnain. (2009). Kultur Jaringan, PT. Bumi Aksara: Jakarta.
Author Biographies
Saipul Sihotang, Universitas Andalas
Renfiyeni Renfiyeni, Universitas Mahaputra Muhammad Yamin
Irfan Suliansyah, Universitas Andalas
Jamsari Jamsari, Universitas Andalas
License
This work is licensed under a Creative Commons Attribution 4.0 International License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under Creative Commons Attribution 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.Penulis.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (Refer to The Effect of Open Access).