DIURETICS EFFECT OF AVOCADO LEAF (Persea americana Mill.) ETHANOL EXTRACT ON THE GASTRIC HISTOLOGY OF WISTAR MALE RATS (Rattus norvegicus)


Dwi Erlinda(1*), Mahriani Mahriani(2), Susantin Fajariyah(3),


(1) Universitas Jember (UNEJ)
(2) Universitas Jember (UNEJ)
(3) Universitas Jember (UNEJ)
(*) Corresponding Author

Abstract


Irregular urine output triggers kidney stone disease. One way to cure it is by using synthetic diuretic compounds. The use of synthetic diuretic drugs for a long time can cause side effects, namely gastric damage. Therefore, research is needed to determine the diuretic effect of ethanol extract of avocado leaves (Persea americana) and its effect on the histological structure of the gastric  of male rats. Avocado leaf extract is made by maceration method, using 70% ethanol and then evaporated. Wistar male rats were divided into 5 treatment groups namely negative control (aquadest), positive control (furosemide), avocado leaf extract dose of 50 mg / kg body weight, 100 mg / kg body weight, and 150 mg / kg body weight with oral administration for 15 days. The volume of urine  was measured for 24 hours, then the mice were sacrified on the 16th day. Gastric organs were taken for histological observation by the paraffin method and Hematoxylin and Eosin (H&E) staining. The results showed that the optimum avocado leaf ethanol extract was a dose of 100 mg/kg showed a diuretic effect but did not affect the histological structure of the gastric.

Keywords


diuretics, ethanol extract of leaves of avocado, gastric histological structure

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References


Adha, A.C. (2009). Pengaruh Pemberian Ekstrak Etanol Daun Alpukat (Persea americana Mill.) Terhadap aktivitas Diuretik Tikus Putih Jantan Sprague-Dawley. Skripsi. Bogor: Fakultas Kedokteran Hewan Institut Pertanian Bogor.

Arukwe, U., Amadi, B.A., Duru, M.K.C., Agomuo,E.N., Adindu, E. A., Odika, P.C., Lele, K.C., Egejuru, L., & Anudike, J. (2012). Chemical Composition of Persea americana Leaf, Fruit and Seed. IJRRAS .11(2):346-349

Bintari, G. S., Windarti, I., & Fiana, D. N.(2014) Temulawak (Curcuma xanthorrhiza Roxb) Sebagai Pencegah Kerusakan Mukosa Lambung. Medical Journal of Lampung University. 3(5) :77-84

Dai J. & Mumper R. (2010). Plant Fenolic : extraction, analysis, and their antioxidant and anticancer properties. Molecules.15(10): 7313-7352

Guyton, A. C., & Hall, J. E., (2014). BukuAjar Fisiologi Kedokteran. Edisi ke-12. Jakarta:EGC Kedokteran.

Katja, D. G., Suryanto, E.,danWehantouw, F., (2009). Potensi daun alpukat (Persea americana Mill.) sebagai sumber antioksidan alami. Chemistry Progress. 2 (10):54-64

Kurniawan, R.F., (2014). Benefits of Avocado Avocado: Treat and Prevent All Diseases. Surabaya: Healthy Books

Mescher, A.L. (2017).Histologi Dasar Junquera Teks & Atlas. Alih Bhs: Jan Tambayong. Edisi 14. Jakarta: Penerbit: EGC

Nessa, Arifin, H., dan Muchtar, H. (2013). Efek Diuretik Dan Daya Larut Batu Ginjal Dari Ekstrak Etanol Rambut Jagung (Zea mays L.). Prosiding Seminar Nasional Perkembangan Terkini Sains Farmasi dan Klinik III 2013: 345-358

Omodamiro, OD & Jimoh, M.A. (2016). Assesment of In-vitro antioxidant and Diuretic potential of an ethanol seed extract of Persea americana in Wistar albino rats. The Pharmaceutical and Chemical Journal. 2016.3(4):134-143

Puspitasari, D. A.(2008). Gambaran Histopatologi Lambung Tikus Putih (Rattus norvegicus) Akibat Pemberian Asam Asetil Salisilat. Skripsi. Bogor: Fakultas Kedokteran Hewan Institut Pertanian Bogor.

Rahman, N., Dewi, N.U., & Bohari. (2018). Phytochemical and Antioxidant Activity of Avocado Leaf Extract (Persea americana Mill.). Asian Journal of Scientific Research. 11 (3): 357-363,

Rostika, N. (2012). Pengaruh Pemberian Ekstrak Minyak Jintan Hitam (Nigella sativa) Terhadap Gambaran Histologi Organ Lambung Dan Usus Halus Mencit (Mus musculus). Skripsi. Bogor: Fakultas Kedokteran Hewan Institut Pertanian Bogor.

Sherwood. (2012). Fisiologi Manusia dari Sel ke Sistem. 6 Ed. Jakarta: Penerbit EGC.

Salawu, O.A., Tijani, A.Y., Obidike, I.C., Rafindadi, H.A., and Emeje, M. (2009). Anti -ulcerogenic properties of methanolic root extract of Piliostigma reticulatum (DC) Hoechst (Syn. Bauhinia reticulate DC) - Leguminosae in rats. African Journal of Pharmacy and Pharmacology. vol. 3(5), pp. 252-258.

Siswandono dan Soekardjo, B.(2008). Kimia Medisinal I. Surabaya: Airlangga University Press.

Wientarsih, I., R. Madyastuti., B. Febran., dan D. Firnanda.(2012). Gambaran Serum Ureum, dan Kreatinin Pada Tikus Putih Yang Diberi Fraksi Etil Asetat Daun Alpukat. Jurnal Veteriner. 13(1):57-62.




DOI: https://doi.org/10.31289/biolink.v7i1.3007

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Fakultas Biologi, Universitas Medan Area

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