EFFECTIVENESS TEST OF ANTI-BACTERIAL LIME JUICE (Citrus aurantifolia) ON SALMONELLA SP BACTERIA IN SALMON FISH (Oncorhynchus nerka)


Qori Fadillah(1*), Linda Chiuman(2), Chrismis Novalinda Ginting(3), Ermi Girsang(4),


(1) Universitas Prima Indonesia
(2) Universitas Prima Indonesia
(3) Universitas Prima Indonesia
(4) Universitas Prima Indonesia
(*) Corresponding Author

Abstract


Salmonella Sp is a gram-negative pathogens that are often found in marine foods,one of  process of serving  salmon is channeling lime juice, where lime juice is acidic and has phytochemical properties that have anti-bacterial properties. This study aims to explore anti-bacterial effect lime juice on Salmonella thypii bacteria in Salmon. This research is an experimental study using  disc diffusion method. Lime samples that were used were purchased from one of the modern markets in Medan which were made in several series of concentrations using distilled water as a solvent, while the salmon samples used in this study were purchased from one of the Japanese dining restaurants located in Medan City. Meanwhile, isolation of Salmonella thypii bacteria from salmon samples was carried out using nutrient broth and SSA media. The results of this study indicate that differences in concentration indicate the differences in the diameter of the inhibitory zone (P value = 0.000). With the diameter of the widest inhibition zone formed by a concentration of 100% is 15.40 ± 0.44 mm and the smallest diameter is formed by a concentration of 5% which is 7.23 ± 0.55 mm. Thus, it can be concluded that the lime juice has anti-bacterial activity against Salmonella thypii bacteria from salmon.

Keywords


Lime juice, Citrus Aurantifolia, Salmonella Sp

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References


Ajizah, A. (2004). Sensitivitas Salmonella Typhimurium Terhadap Ekstrak Daun Psidium Guajava L . Bioscientiae, 1(1), 31–38.

Benigna, M. (2015). Uji Daya Hambat Ekstrak Daun Keji Beling (Srobilanthes Crispa Bl.) Terhadap Pertumbuhan Bakteri Salmonella Typhi Secara In Vitro. Sanata Dharma University. Diambil dari https://repository.usd.ac.id/981/

BPOM RI. (2014). Keracunan Pangan Akibat Bakteri Patogen Bag.II. Sentra Informasi Keracunan Nasional : Badan Pengawas Obat dan Makanan (BPOM) RI.

Fajarwati, N. (2013). Uji aktivitas antioksidan pada ekstrak daun jeruk nipis (Citrus aurantifolia) dengan menggunakan metode dpph (1, 1-diphenyl-2-2-picrylhydrazyl). UIN Syarif Hidayatullah. Jurnal kesehatan dan kedokteran, 2015

Khanifah, F. (2015). Efek Pemberian air perasan jeruk nipis (Cirus aurantifolia (Christm) Swingle) Terhadap Pembentukan, Pertumbuhan Dan Penghancuran Biofilm staphylococcus aureus Secara In Vitro. UIN Syarif Hidyatullah Jakarta. Jurnal kesehatan dan kedokteran, 2015.

Liew, S. S., Ho, W. Y., Yeap, S. K., & Sharifudin, S. A. Bin. (2018). Phytochemical composition and in vitro antioxidant activities of Citrus sinensis peel extracts. PeerJ, Vol 6. National Library of Medicine Journal e5331–e5331. https://doi.org/10.7717/peerj.5331

Menteri Kesehatan. Peraturan Menteri Kesehatan Republik Indonesia Tentang Higiene Sanitasi Jasaboga (2011). Indonesia.

Miguéis, S., Moura, A. T., Saraiva, C., & Esteves, A. (2016). Influence of season and type of restaurants on sashimi microbiota. European Journal of Public Health, 26(5), 877–881. https://doi.org/10.1093/eurpub/ckw009

Morgan, D. (2005). Salmon. San Francisco: Chronicle Books.

Olaimat, A. N., & Holley, R. A. (2013). Effects of changes in pH and temperature on the inhibition of Salmonella and Listeria monocytogenes by Allyl isothiocyanate. Food Control, 34(2), 414–419. https://doi.org/10.1016/j.foodcont.2013.05.014

Rahayu, W. P. (2000). Aktivitas antimikroba bumbu masakan tradisional hasil olahan industri terhadap bakteri patogen dan perusak. Bul. Teknol. dan Industri Pangan, XI(2), 42–48.

Razak, A., Djamal, A., & Revilla, G. (2013). Uji Daya Hambat Air Perasan Buah Jeruk Nipis (Citrus aurantifolia s .) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus Secara In Vitro. Jurnal Kesehatan Andalas, 2(1), 5–8.

Sati, P., Dhyani, P., Bhatt, I. D., & Pandey, A. (2019). Ginkgo biloba flavonoid glycosides in antimicrobial perspective with reference to extraction method. Journal of Traditional and Complementary Medicine, 9(1), 15–23. https://doi.org/10.1016/j.jtcme.2017.10.003

Schwarz, S., Silley, P., Simjee, S., Woodford, N., van Duijkeren, E., Johnson, A. P., & Gaastra, W. (2010). Assessing the antimicrobial susceptibility of bacteria obtained from animals. Veterinary Microbiology, 65(4), 601–604. https://doi.org/10.1016/j.vetmic.2009.12.013

Suandy, Girsang, E., Nasution, A. N., & Lister, I. N. E. (2020). Analysis of Protein Content, Spectrophotometry FT-IR, and Antibacterial Effects of Earthworm (Eudriluseugenia). American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS), 63(1), 94–101.

Sumono, A., & SD, A. W. (2008). The use of bay leaf (Eugenia polyantha Wight) in dentistry. Dental Journal (Majalah Kedokteran Gigi), 41(3), 147–150. https://doi.org/10.20473/j.djmkg.v41.i3.p147-150

Weinstein, M. P., Patel, J. B., Bobenchik, A. M., Campeau, S., Cullen, S. K., Galas, M. F., … Tamma, P. D. (2019). M100 Performance standards for antimicrobial susceptibility testing. Clinical Microbiology Newsletter (29th ed.). PA: Clinical and Laboratory Standards Institute. https://doi.org/10.1016/s01964399(01)88009-0

Yahya, H. (2016). Pengaruh Air Perasan Buah Jeruk Nipis (Citrus Aurantifolia Swingle) Terhadap Hambatan Pertumbuhan Bakteri Enterococcus Faecalis Dominan Pada Saluran Akar Secara In Vitro. Thesis Universitas Muhammadiyah Surakarta.




DOI: https://doi.org/10.31289/biolink.v7i1.3322

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Fakultas Biologi, Universitas Medan Area

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