Skrining Fitokimia dan Antimikroba Ekstrak Daun Kirinyuh terhadap Bakteri Staphylococcus Aureus Dan Escherichia Coli

Nuriana Munte(1*), Sartini sartini(2), Rosliana Lubis(3),


(1) Fakultas Medan Area
(2) Universitas Medan Area
(3) Universitas Medan Area
(*) Corresponding Author

Abstract


This reseach aims to determine the composition of secondary metabolites of kirinyuh leaf extract (Euphatorium ordoratum.L) and antimicrobial properties of the extract against Staphylococcus aureus and Escherichia coli. The study was conducted in three phases: the first phase is manufacture kirinyuh leaf extract (Euphatprium odoratum L) through maceration method using N-Hexane solvent and methanol. In stage II, phytochemical screening, namely the identification of secondary metabolites (alkaloids, flavonoids, terpenoids and steroids) to the crude extract of  leaves kirinyuh (Ephatorium odoratum L) in the form of antimicrobial properties of extracts against Staphylococcus aureus and with variations extract concentrations of 0%, 1 %, 5%, 10%, 15%, 20%, and 25% Echerichia coli and incubated at 37°C for 24 hours. The research found that such compounds secondary metbolit alkaloids, flavonoids, steronoid, and saponin, which dominates the secondary metabolites of four that alkaloid. While the screening test of phytochemical N-hexane extracts is not contain metbolit secondary compounds, testing against bacteria possess bioktivitas inhibition at concentrations of 15% with inhibition zone of 1.3 cm on Escherichia coli bacteria and 1.0 cm in bacteria Staphylococcus aureas. This shows kirinyuh leaf extracts have the ability as an anti-microbial.

Keywords


extract leaf kirinyuh, Staphylococcus aureus , Echerichia coli

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References


Afrianti, R, R. Yenti, dan L. Afriani, 2010., Studi pendahuluan etanol daun kirinyuh terhadap penyembuhan luka, Laporan Penelitian STIFL, Padang.

Armando Caceres., Herlinda Menendez., Emilia Mendez., Ericka Cohoboan., Blanca E. Samayoa., Elsa Jauregui et al., 1995. Antigonorrhoeal activity of Plants Used in Guatemala for the reatment of Sexually Transmitted Diseses. J. Ethopharmacol, 48 (2) : 85 : 88.

Awoyinka, O.A., Balogun, I. O,. Ongunnowo, A.A., 2007, Phytochemacal Screening and in Vitro Bioactivity Cnidoscolus acounitifolius (Euphorbiaceae), J. of Medicinal Plants Res., 1 (3) : 063- 065.

Benjamin, V.T, et.al., 1987., Phytochemical and antibacterial Strudies on The Essential Oil of Euphatorium Odoratum, Available online at http://www.pharmaceutical Biology, diakses : 24 februari 2013.

Harborne, J. B. 1996 Metide Fitokimia. Bandung : Institut Teknologi Bandung

Inya-agha, S.I., B.O Oguntimein., A. Sofowora and T. C. Benjamin, 1987, phyochemical and Antibacterial studies on the Essential oil of Euphatorium odoratum L. Pharmaceutical biology, (1) vol. 25, pages 49-52, Departemen of Pharmacognosy, School of Pharmacy, University of lagos, Nigeria

Trobi, O.N., 1997, Antibiotic Properties of Ethanol Extract of Chromolaena Odorata (Astraceane). International Journal of Pharmacognosy, 35():111-115.

James A. Duke SA, Edword SA, 1989, Medicinal Plants of China, 4th ed. Michigan, in the United States of America.

Jane R.M.R., Albuquerqu, Edilberto R. Silveira, Daniel Esdras De A. Uchon. Et al and Otilia Deusdenia L. pessoa, 2004,Chemicel Composition andLarvicidal Activity of the Essential

Oils from Euphatorium betonicaeforme (D.C) Baker (Asteraceae), J. Agric. Food Chem, 52 (22), PP 6708-7611

Owolabi, M.S., Akintayo Ogundajo., Kamil O. Yusuf., Labunmi Lajide., Heather E. Villanueva., Jessika A. Tuten., and William N. Setzer., 2010, Chemical Composition and Bioactivity of the Essential Oil of Chromolaena odorata fron Nigeria, ACG publication, Records Natural products, 4:172-78.

Purwati, 2003, Isolasi dan Identifikasi Senyawa Organik Dalam daun Euphatorium odoratum L., tesis, jurusan kimia, FMIPA, Universitas Gadjah Mada, Yogyakarta.

Purwati dan Undri R. 2009. Skrining Senyawa Metabolit Sekunder dan Uji Aktivitas Antioksidan Ekstrak Etilasetat Daun Wedusan (Euphatorium odoratum) Molekul, program studi kimia, jurusan MIPA, Fakultas Sains dan Teknik UNSOED vol. 4.No. 2. November, 2009:94-104.

Rungnapa, O,.2003, Phytochemistry and Antimalarial Activity of Euphatorium odoratum L., Thesis, Pharmaceutical Chemistry And phytochemstry, Faculty of Graduate Studies, Mahidol University, Bangkok.

Saiful, 2005, Isolasi dan Identifikasi Senyawa Antimikroba Dari Daun Galinggang (Cassia alata linn). Tesis, Universitas Gadjah Mada. Yogyakarta.

Thakong, K., 1999, A Study on the Antimalasarial Constituests and Chemical Chemistry and Phytochermistry) Facultyof Gradute Studies Studies, Mahidol University, Bangkok

Vital, P. G., and W. L, Rivera, 2009.Antimicrobacterial activity and citoxicity of chromolaena odorator (L.F) King and Robinson and Uncaria perrottetii (A. rich) merr.Extracts, Available online at http;//www.academicjournal.org/JMPR Journal of Medicial Plant Research vol. 3 (7), pp. 5511-518.




DOI: http://dx.doi.org/10.31289/biolink.v2i2.803

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