ISOLASI DAN KARAKTERISASI BAKTERI RESISTEN TEMBAGA DARI PANTAI TIMUR SURABAYA

Autor(s): Wahyu Irawati
DOI: 10.31289/biolink.v6i2.2558

Abstract

Copper pollution in the East Coast of Surabaya is one of the serious cases of heavy metal pollution in Indonesia. Pollution at this location has been proven to result in fish deaths and brain damage of local residents because they consume too much copper-contaminated fish. Copper bioremediation using indigen bacteria isolated from polluted environments is a promising solution to overcome copper pollution problems. Bacteria that commonly live in polluted environments can be isolated and used as copper bioremediation agents. This study aims to do: 1) isolation and characterization of copper resistant bacteria from the East Coast of Surabaya, 2) resistance testing of bacterial isolates, and 3) copper accumulation and biosorbtion tests of bacterial isolates. The bacterial isolates were characterized by the morphology of the colonies and their cells based on Gram staining. Resistance testing is done by determining Minimum Inhibitory Concentration/MIC. The accumulation test is carried out by separating the cell and growth medium, then each of them is distructed using HNO3. The results of isolation and characterization obtained six isolates of copper-resistant bacteria, namely PmbC1, PmbC2, PmbC3, PmbC4, PmbC5, and PmbC6 with MIC = 3 mM - 5mM CuSO4. PmbC4 isolate is the most resistant bacteria with the MIC of 5 mM and is capable of accumulating copper of 6.25 mg per gram of cell dry weight and biosorbtion of 92.17%.

Keywords

Bacteria, Resistant, Copper, Accumulation, Biosorbtion

Full Text:

PDF

References

Ahemad M. (2012). Implication of bacterial resistance against heavy metals in bioremediation: a review. J Inst Integrat Omics Appl Biotechnol 3(3): 39-46.

Ansari, M.I., Masood, F. & Malik, A. (2011). Microbes and Microbial Technilogy.New York: Springer.

Arisandi, P. (2013). The Rufford Small Grants for Nature Conservation. Disadur dari: http://www.rufford.org/rsg/projects/prigi_arisandi (1 Mei 2013).

Babák, L., Šupinová, P., Zichová, M., Burdychová, R. & Vítová, E. (2012). Biosorption of Cu, Zn and Pb by thermophilic bacteria – effect of biomass concentration on biosorption capacity. Acta Universitatis Agricultureae et Silviculturae Mendelianae Brunensis, 60: 9-18.

Bondarczuk K, Piotrowska-Seget Z. (2013). Molecular basis of active copper resistance mechanisms in Gram-negative bacteria. Cell Biol Toxicol 29: 397-405.

Irawati, W., Ompusunggu, N. P., Susilowati, D. N., & Yuwono, T. (2019). Molecular and physiological characterization of indigenous copper resistant bacteria from Cikapundung River, West Java, Indonesia. Biodiversitas, 20(2): 344-349.

Irawati, W., Yuwono, T., Soedarsono, J. & Hartiko, H. (2012). Molecular and Physiological Characterization of Copper-Resistant Bacteria Isolated from Activated Sludge in an Industrial Wastewater Treatment Plant in Rungkut Surabaya, Indonesia. Microbiology Indonesia, 6: 107-116.

Kotrba, P. (2011). Microbial Biosorption of Metals. Berlin: Springer.

Madigan, M.T., Martinko, J.M., Dunlap, P.V. & Clark, D.P. (2009). Biology of Mocrobiology, Twelfth Edition. San Fransisco: Pearson Benjamin Cummings.

Mulyadi, E., Laksmono, R. & Aprianti, D. (2011). Fungsi Mangrove sebagai Pengendali Pencemar Logam Berat. Jurnal Ilmiah Teknik Lingkungan Edisi Khusus. 1: 33-40.

Rosahada, A. D., Budiyono, & Dewanti, N. A. (2018). Biokonsentrasi Logam Berat Tembaga (Cu) dan Pola Konsumsi Ikan Mujair di Wilayah Danau Rawapening. Jurnal Kesehatan Masyarakat. 6(6):1-7.

Rolfe, M. D., Rice, C. J., Lucchini, S., Pin, C., Thompson, A., Cameron, A. D. S., Alston, M., Stringer, M. F., Betts, R. P., Baranyi, J., Peck, M. W. & Hinton, J. C. D. (2012). Lag Phase Is a Distinct Growth Phase That Prepares Bacteria for Exponential Growth and Involves Transient Metal Accumulation. Journal of Bacteriology, 194:686-701.

Santo, C. E., Morais, P. V. & Grass, G. (2010). Isolation and characterization of bacteria resistant to metallic copper surfaces. Applied and Environmental Microbiology, 76: 1341 – 1348

Shetty, R. & Rajkumar, Sh. (2009). Biosorption of Cu (II) by Metal Resistant Pseudomonas sp. International Journal of Environmental Research, 3: 121-128

Starr, T. J. & Jones, M. E. (1957). The effect of copper on the growth of bacteria isolated from marine environments. Limnology and Oceanography, 2:33-36.

Yi, Q. (2009). Point Sources of Pollution: Local Effects and It’s Control. Oxford: Eolss Publishers Company Limited.

Zaidi, A., Wani, P.A. & Khan, M.S. (2012). Toxicity of Heavy Metals to Legumes and Bioremediation. Pondicherry: Springer.

Refbacks

  • There are currently no refbacks.